For some time I have been using a small app called What’s Keeping Me to find out what process is locking a file. And it’s free!
About What’s Keeping Me?
Have you ever have the problem where you can’t empty the Trash or eject a disk because something is preventing you? Usually the reason is because some application has a file open, and thus you can’t get rid of the disk or trash the file. That’s why we made What’s Keeping Me! What’s Keeping Me will identify the application that is holding the item open. You can then use What’s Keeping Me to quit the problem application (or kill it if needed) so you can perform your task. What’s Keeping Me includes an Automator workflow so you can perform searches directly from the Finder too!